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The cryopreservation of canine semen can be performed utilizing either the small 0.5ml straws (desireable when the semen will be used for surgical or transcervical insemination by direct injection into the uterus), or the larger 5ml "Macrotubes" we commonly use for equine semen (preferable for vaginal AI). A minimum dose is generally considered to be 100 million, progressively motile cells, whether the semen is fresh or frozen-thawed. With frozen semen, therefore, we usually aim for about 400 million total cells in a dose to begin with. At a "minimum" postthaw motility of 30%, this will give us 120 million motile cells for the resulting insemination.

Dogs produce between one and about 15 straws per collection, depending upon age, breed, and health status. Smaller dogs produce less than larger dogs, and as with any species, younger adults produce the best quality samples. We normally test four different extenders at first, one of which we have developed ourselves. This "test-freeze" determines which extender we use in subsequent freezing sessions.  For most males, collection once per week provides optimal results for both semen quality and quantity.  While a teaser female is rarely required, we seem to obtain more sperm when one happens to be "handy".

Any surgical insemination work is performed a few miles away in a colleague's surgical suite, although this method is being rapidly replaced by the transcervical procedure using endoscopic visualization of the cervix, which we perform and recommend. It is quicker, less expensive, less risky, and less stressful, with similar pregnancy rates.

We have also developed a shipping extender for fresh-chilled semen that maintains exceptional longevity; in some cases over several weeks! Because we make these extenders ourselves, from the very finest chemicals available, and are not required to use proprietary media sold other networks, we can offer semen cryopreservation to the breeder at an extremely competitive fee.

We have been inspected by the AKC and are officially approved by them as a semen collection center.

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Last modified: 06/08/08